Badger酒精液体模型饲料
Badger Ethanol Liquid Diet
一、Badger Ethanol Liquid Diet的原来与用途
【1】酒精对生理机能以及妊娠、胎儿的影响；
【2】任何酒精性疾病的造模；
【3】酒精毒性和毒理学的研究；
【4】药物、食品功能因子、保健品对酒精的拮抗作用；
【5】疾病造模后药物、食品功能因子、保健品的治疗或者辅助治疗效果及其机制；
【6】毒物与酒精在机体的相互作用。的相互作用。
Badger酒精液体模型饲料的特点：【1】可以按研究需要任意确定动物所要达到的血液酒精浓度；
【2】按照标准的肠内营养方法（TEN Model）给予饲料；
【3】饲料配比与人类肠内营养饮食相似。
Badger等学者专注于研究肠内营养（ total enteral nutrition，TEN）并使用TEN法（TEN Model）研究了酒精对实验动物的生理机能的影响和疾病造模。
由于采用了TEN的方法给予酒精液体饲料，任何自由喂养所不能达到的血液酒精浓度，在本法中都能达到，原因是给予饲料的方法与Tsukamoto-French模型相似。
TP5500：Badger酒精液体模型饲料，脂肪17.5%, 蛋白质15%, 碳水化合物32.5%, 酒精35%。
TP5500C：TP5500的对照饲料，脂肪17.5%, 蛋白质15%, 碳水化合物67.5%。
TP5501：Badger高脂低碳水化合物酒精液体模型饲料，脂肪25%, 蛋白质16%, 碳水化合物21%, 酒精38%。
TP5501C：TP5501的对照饲料，脂肪25%, 蛋白质16%, 碳水化合物59%。
TP5502：Badger高脂低碳水化合物酒精液体模型饲料，脂肪45%, 蛋白质16%, 碳水化合物2.5%, 酒精36.5%。
TP5502C：TP5502的对照饲料，脂肪45%, 蛋白质16%, 碳水化合物39%。
你还可以根据研究需要定制任意组成！
Badger酒精液体饲料可以看作是Lieber-DeCarli酒精液体饲料基础上改进的模型饲料，也可以根据需要任意调整蛋白质、碳水化合物和脂肪的热量比例，或者任意更改任何营养素的含量（缺乏、过载）。
因此，Badger酒精液体饲料使用的关键是，需要预先对动物胃内（或食管）埋管。其实，做过这种方法的研究人员都体会到该方法并不复杂，难度不大。
二、Badger Ethanol Liquid Diet应用的注意点
1.购买饲料前：
（1）应当做好动物的准备，注意动物的品种和品系的选择。
（2）胃管和手术的相关准备。
（3）饲料喂养流程设计。
2. 购买后应当向公司索取以下资料：
《液体饲料配制方法》
《酒精液体饲料喂养方法》
《怎样根据研究的需要调整酒精的配比》
References：
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Shankar K, Ronis MJ, Badger TM. Effects of pregnancy and nutritional status on alcohol metabolism. Alcohol Res Health. 2007;30(1):55-9.
Ronis MJ, Lumpkin CK, Ingelman-Sundberg M, Badger TM. Effects of short-term ethanol and nutrition on the hepatic microsomal monooxygenase system in a model utilizing total enteral nutrition in the rat. Alcohol Clin Exp Res. 1991 Aug;15(4):693-9.
Shankar K, Hidestrand M, Liu X, Xiao R, Skinner CM, Simmen FA, Badger TM, Ronis MJ. Physiologic and genomic analyses of nutrition-ethanol interactions during gestation: Implications for fetal ethanol toxicity. Exp Biol Med (Maywood). 2006 Sep;231(8):1379-97.
Baumgardner JN, Shankar K, Korourian S, Badger TM, Ronis MJ. Undernutrition enhances alcohol-induced hepatocyte proliferation in the liver of rats fed via total enteral nutrition. Am J Physiol Gastrointest Liver Physiol. 2007 Jul;293(1):G355-64.
Ronis MJ, Butura A, Korourian S, Shankar K, Simpson P, Badeaux J, Albano E, Ingelman-Sundberg M, Badger TM. Cytokine and chemokine expression associated with steatohepatitis and hepatocyte proliferation in rats fed ethanol via total enteral nutrition. Exp Biol Med (Maywood). 2008 Mar;233(3):344-55.
Baumgardner JN, Shankar K, Hennings L, Badger TM, Ronis MJ. A new model for nonalcoholic steatohepatitis in the rat utilizing total enteral nutrition to overfeed a high-polyunsaturated fat diet. Am J Physiol Gastrointest Liver Physiol. 2008 Jan;294(1):G27-38.
Ronis MJ, Hennings L, Stewart B, Basnakian AG, Apostolov EO, Albano E, Badger TM, Petersen DR. Effects of long-term ethanol administration in a rat total enteral nutrition model of alcoholic liver disease. Am J Physiol Gastrointest Liver Physiol. 2011 Jan;300(1):G109-19.
Ronis MJ, Butura A, Sampey BP, Shankar K, Prior RL, Korourian S, Albano E, Ingelman-Sundberg M, Petersen DR, Badger TM. Effects of N-acetylcysteine on ethanol-induced hepatotoxicity in rats fed via total enteral nutrition. Free Radic Biol Med. 2005 Sep 1;39(5):619-30.
Baumgardner JN, Shankar K, Hennings L, Albano E, Badger TM, Ronis MJ. N-acetylcysteine attenuates progression of liver pathology in a rat model of nonalcoholic steatohepatitis. J Nutr. 2008 Oct;138(10):1872-9.
Ronis MJ, Korourian S, Blackburn ML, Badeaux J, Badger TM. The role of ethanol metabolism in development of alcoholic steatohepatitis in the rat. Alcohol. 2010 Mar;44(2):157-69. doi: 10.1016/j.alcohol.2009.11.002.
Ronis MJ, Korourian S, Zipperman M, Hakkak R, Badger TM. Dietary saturated fat reduces alcoholic hepatotoxicity in rats by altering fatty acid metabolism and membrane composition. J Nutr. 2004 Apr;134(4):904-12.
Badger TM, Crouch J, Irby D, Hakkak R, Shahare M. Episodic excretion of ethanol during chronic intragastric ethanol infusion in the male rat: continuous vs. cyclic ethanol and nutrient infusions. J Pharmacol Exp Ther. 1993 Feb;264(2):938-43.
Ronis MJ, Huang J, Crouch J, Mercado C, Irby D, Valentine CR, Lumpkin CK, Ingelman-Sundberg M, Badger TM. Cytochrome P450 CYP 2E1 induction during chronic alcohol exposure occurs by a two-step mechanism associated with blood alcohol concentrations in rats. J Pharmacol Exp Ther. 1993 Feb;264(2):944-50.
Badger TM, Ronis MJ, Ingelman-Sundberg M, Hakkak R. Pulsatile blood alcohol and CYP2E1 induction during chronic alcohol infusions in rats. Alcohol. 1993 Nov-Dec;10(6):453-7.
Albano E, Clot P, Morimoto M, Tomasi A, Ingelman-Sundberg M, French SW. Role of cytochrome P4502E1-dependent formation of hydroxyethyl free radical in the development of liver damage in rats intragastrically fed with ethanol. Hepatology 23: 155–163, 1996.
Badger TM, Ronis MJ, Lumpkin CK, Valentine CR, Shahare M, Irby D, Huang J, Mercado C, Thomas P, Ingelman-Sundberg M, Crouch J. Effects of chronic ethanol on growth hormone secretion and hepatic cytochrome P450 isozymes of the rat. J Pharmacol Exp Ther 264: 438–447, 1993.
He L, Ronis MJJ, Badger TM. Ethanol induction of class I alcohol dehydrogenase expression in the rat occurs through alterations in CCAAT/enhancer binding proteins β and γ. J Biol Chem 277: 43572–43577,
Korourian S, Hakkak R, Ronis MJ, Shelnutt SR, Waldron J, Ingelman-Sundberg M, Badger TM. Diet and risk of ethanol-induced hepatotoxicity: carbohydrate-fat relationships in rats. Toxicol Sci 47: 110–117, 1999.
Ronis MJ, Ingelman-Sundberg M, Badger TM. Induction, suppression and inhibition of multiple hepatic cytochrome P450 isozymes in the male rat and bobwhite quail (Colinus virginianus) by ergosterol biosynthesis inhibiting fungicides (EBIFs). Biochem Pharmacol 48: 1953–1965, 1994.
Ronis MJJ, Korourian S, Zipperman M, Hakkak R, Badger TM. Effects of dietary saturated fat and ethanol on growth and hepatotoxicity in rats fed via total enteral nutrition. J Nutr 134: 904–912, 2004.
Ronis MJJ, Korourian S, Yoon S, Ingelman-Sundberg M, Albano E, Lindros K, Badger TM. Lack of sexual dimorphism in alcohol-induced liver damage (ALD) in rats treated chronically with ethanol-containing low carbohydrate diets: the role of ethanol metabolism and endotoxin. Life Sciences 75: 469–483, 2004.
Chen JR, Lazarenko OP, Shankar K, Blackburn ML, Badger TM, Ronis MJ. A role for ethanol-induced oxidative stress in controlling lineage commitment of mesenchymal stromal cells through inhibition of Wnt/beta-catenin signaling. J Bone Miner Res. 2010 May;25(5):1117-27.
Chen JR, Lazarenko OP, Shankar K, Blackburn ML, Lumpkin CK, Badger TM, Ronis MJ. Inhibition of NADPH oxidases prevents chronic ethanol-induced bone loss in female rats. J Pharmacol Exp Ther. 2011 Mar;336(3):734-42.
Chen JR, Shankar K, Nagarajan S, Badger TM, Ronis MJ. Protective effects of estradiol on ethanol-induced bone loss involve inhibition of reactive oxygen species generation in osteoblasts and downstream activation of the extracellular signal-regulated kinase/signal transducer and activator of transcription 3/receptor activator of nuclear factor-kappaB ligand signaling cascade. J Pharmacol Exp Ther. 2008 Jan;324(1):50-9.
Shankar K, Hidestrand M, Liu X, Chen JR, Haley R, Perrien DS, Skinner RA, Lumpkin CK Jr, Badger TM, Ronis MJ. Chronic ethanol consumption inhibits postlactational anabolic bone rebuilding in female rats. J Bone Miner Res. 2008 Mar;23(3):338-49.
Badger TM, Ronis MJ, Ingelman-Sundberg M, Hakkak R. Inhibition of CYP2E1 activity does not abolish pulsatile urine alcohol concentrations during chronic alcohol infusions. Eur J Biochem. 1995 Jun 15;230(3):914-9.
Hu Y, Mishin V, Johansson I, von Bahr C, Cross A, Ronis MJ, Badger TM, Ingelman-Sundberg M. Chlormethiazole as an efficient inhibitor of cytochrome P450 2E1 expression in rat liver. J Pharmacol Exp Ther. 1994 Jun;269(3):1286-91.
Ronis MJ, Huang J, Longo V, Tindberg N, Ingelman-Sundberg M, Badger TM. Expression and distribution of cytochrome P450 enzymes in male rat kidney: effects of ethanol, acetone and dietary conditions. Biochem Pharmacol. 1998 Jan 15;55(2):123-9.
Hu Y, Ingelman-Sundberg M, Lindros KO. Induction mechanisms of cytochrome P450 2E1 in liver: interplay between ethanol treatment and starvation. Biochem Pharmacol. 1995 Jul 17;50(2):155-61.
Fang C, Lindros KO, Badger TM, Ronis MJ, Ingelman-Sundberg M. Zonated expression of cytokines in rat liver: effect of chronic ethanol and the cytochrome P450 2E1 inhibitor, chlormethiazole. Hepatology. 1998 May;27(5):1304-10.
Brown EC, Perrien DS, Fletcher TW, Irby DJ, Aronson J, Gao GG, Hogue WJ, Skinner RA, Suva LJ, Ronis MJ, Hakkak R, Badger TM, Lumpkin CK Jr. Skeletal toxicity associated with chronic ethanol exposure in a rat model using total enteral nutrition. J Pharmacol Exp Ther. 2002 Jun;301(3):1132-8.
Wahl EC, Liu L, Perrien DS, Aronson J, Hogue WR, Skinner RA, Hidestrand M, Ronis MJ, Badger TM, Lumpkin CK Jr. A novel mouse model for the study of the inhibitory effects of chronic ethanol exposure on direct bone formation. Alcohol. 2006 Jul;39(3):159-67.
He L, Simmen FA, Mehendale HM, Ronis MJ, Badger TM. Chronic ethanol intake impairs insulin signaling in rats by disrupting Akt association with the cell membrane. Role of TRB3 in inhibition of Akt/protein kinase B activation. J Biol Chem. 2006 Apr 21;281(16):11126-34.
He L, Simmen FA, Ronis MJ, Badger TM. Post-transcriptional regulation of sterol regulatory element-binding protein-1 by ethanol induces class I alcohol dehydrogenase in rat liver. J Biol Chem. 2004 Jul 2;279(27):28113-21.
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可以任意缺乏或过载某营养素
可以任意添加药物或测试成分
可以精确定量饲料摄入量
可以任意定制饲料
- AIN76酒精液体饲料(TP4000)
- AIN93G酒精液体饲料(TP4010)
- AIN93M酒精液体饲料(TP4020)
- 标准型Lieber-DeCarli酒精液体饲料(TP4030)
- 43%高脂肪型Lieber-DeCarli酒精液体饲料(TP4039)
- 12%低脂型Lieber-DeCarli酒精液体饲料(TP4032)
- 10%低脂肪型Lieber-DeCarli酒精液体饲料(TP4031)
- 5%低脂型Lieber-DeCarli酒精液体饲料(TP4033)
- 2%低脂型Lieber-DeCarli酒精液体饲料(TP4034)
- 25%高蛋白型Lieber-DeCarli酒精液体饲料(TP4035)
- 12.5%低蛋白型Lieber-DeCarli酒精液体饲料(TP4036)
- 4%低蛋白型Lieber-DeCarli酒精液体饲料(TP4038)
- 2%低蛋白型Lieber-DeCarli酒精液体饲料(TP4037)
- 26%酒精Rao-Larkin酒精液体饲料(TP4063)
- 40%酒精高脂Nanji液体饲料(TP4054)
- 29%酒精高脂Nanji液体饲料(TP4062)
- Lindros酒精液体饲料(TP4060)
- Lieber-DeCarli酒精液体饲料25%高蛋白型
- Weinberg-Keiver酒精液体饲料(TP4040)
- Yeh-Cerklewski酒精液体饲料(TP4100)
- Sahchis酒精液体饲料(TP4230)
- Wiener酒精液体饲料(TP4200)
- Tsukamoto-French模型酒精液体饲料
- Thompson-Reitz酒精液体模型饲料(TP5100)
- Badger酒精液体饲料
- 微型猪（小型猪）酒精液体饲料
- 灵长类Lieber-DeCarli酒精液体饲料(TP7000)