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TP4062:Tipoe-Nanji酒精液体模型饲料(29%酒精型)
Tipoe-Nanji Ethanol Liquid Diet(Nanji diet,29%ethanol)

一、Tipoe-Nanji diet with 29% alcohol的由来和特点


特洛菲饲料科技有限公司
Tipoe-Nanji-29%酒精液体饲料
【1】模型饲料:

南通特洛菲饲料科技有限公司饲料代码:TP4062;

脂肪35%, 蛋白质36%, 酒精29%(4.5% v/v)。

配制1升(1000毫升)需要饲料149.8g,鱼油41.8g,添加95%酒精或无水酒精。配成液体后每毫升1千卡。

【2】对照饲料:

南通特洛菲饲料科技有限公司饲料代码:TP4062C;

脂肪35%, 蛋白质36%, 碳水化合物29%。

配制1升(1000毫升)需要饲料178.2g,鱼油41.8g,添加95%酒精或无水酒精。配成液体后每毫升1千卡。

Tipoe-Nanji diet当前有两种常用的酒精液体模型饲料,一种是酒精占40%的酒精液体饲料,另一种是下面要介绍的酒精占29%的酒精液体饲料,两种模型饲料都是以鱼油作为主要脂肪原料。40%酒精液体饲料首先被创建(务必点击了解),然后出现了29%酒精液体饲料。

文献中把这两种饲料都成为Nanji diet,而实际上,从相关文献分析,Tipoe对这两种模型饲料的贡献起了重要作用,因此,我们这里称为Tipoe-Nanji diet。

毫无疑问,29%酒精的Tipoe-Nanji酒精液体饲料提供的酒精要比40%酒精的要低得多。然而,在喂养大鼠和小鼠的效果方面,目前还没有资料说明有什么差别,比如,都能引起酒精性脂肪肝,肠粘膜通透性增加,内毒素血症,等等。

尽管如此,效应的差异性一定是有的,因为两种饲料之间有两个最为关键的区别,一是,29%酒精型几乎没有碳水化合物。以往的研究中已经知道,如果酒精液体饲料中碳水化合物过低时,肝脏糖代谢异常,ATP生成减少(减慢)。二是,29%酒精型酒精含量低,能够推测到血液酒精水平可能较低,这就可以推测作用比较温和,因此,特别适合慢性肝内、肝外组织或器官的毒性研究。

然而,29%酒精型的优势正是因为酒精含量低,使得动物容易接受。试想,鱼油的味道是难以接受的,再让动物饮酒,确实让为我们的研究做出牺牲的大小鼠们为难了。

Tipoe-Nanji 29%酒精液体模型饲料的用途和特点
Tipoe-Nanji酒精液体模型饲料的用途:主要是:

【1】酒精性肝病、酒精性骨质疏松症等疾病的造模和机制的研究;

【2】在酒精性肝病造模中,不仅有脂肪肝,而且可伴有炎症、坏死、ALT升高,内毒素血症;

【2】药物、食品功能因子、保健品对酒精或酒精性疾病的拮抗作用;

【3】饮食不同脂肪或不同脂肪酸构成与酒精在酒精毒性或酒精性疾病中的相互作用的研究;

Tipoe-Nanji酒精液体模型饲料的特点:

【1】高脂肪、低碳水化合物;

【2】脂肪以鱼油为主;

【3】自由喂养。

 

二、Tipoe-Nanji diet with 29% alcoho应用的注意点


1.购买饲料前:

(1)由于含量大量的鱼油,应当准备4度存放饲料的条件。

(2)应当做好动物的准备,注意动物的月龄、体重,特别注意动物的品种和品系的选择。

(3)务必熟知饲料喂养流程。

2. 购买手续完成后应当向公司索取以下资料:

《液体饲料配制方法》

《酒精液体饲料喂养方法》

《怎样根据研究的需要调整酒精的配比》

References:

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Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Thomas P, Dannenberg AJ. Curcumin prevents alcohol-induced liver disease in rats by inhibiting the expression of NF-kappa B-dependent genes. Am J Physiol Gastrointest Liver Physiol. 2003 Feb;284(2):G321-7.

Nanji AA, Jokelainen K, Tipoe GL, Rahemtulla A, Dannenberg AJ. Dietary saturated fatty acids reverse inflammatory and fibrotic changes in rat liver despite continued ethanol administration. J Pharmacol Exp Ther. 2001 Nov;299(2):638-44.

Summa KC, Voigt RM, Forsyth CB, Shaikh M, Cavanaugh K, Tang Y, Vitaterna MH, Song S, Turek FW, Keshavarzian A. Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and Inflammation. PLoS One. 2013 Jun 18;8(6):e67102. Print 2013.

Tipoe GL, Liong EC, Casey CA, Donohue TM Jr, Eagon PK, So H, Leung TM, Fogt F, Nanji AA. A voluntary oral ethanol-feeding rat model associated with necroinflammatory liver injury. Alcohol Clin Exp Res. 2008 Apr;32(4):669-82.

Forsyth CB, Tang Y, Shaikh M, Zhang L, Keshavarzian A (2011) Role of snail activation in alcohol-induced iNOS-mediated disruption of intestinal epithelial cell permeability. Alcohol Clin Exp Res 35: 1635–1643.

Nanji AA, Zhao S, Sadrzadeh SM, Dannenberg AJ, Tahan SR, Waxman DJ. Markedly enhanced cytochrome P450 2E1 induction and lipid peroxidation is associated with severe liver injury in fish oil-ethanol-fed rats. Alcohol Clin Exp Res. 1994 Oct;18(5):1280-5.

Morimoto M, Zern MA, Hagbjörk AL, Ingelman-Sundberg M, French SW. Fish oil, alcohol, and liver pathology: role of cytochrome P450 2E1. Proc Soc Exp Biol Med. 1994 Nov;207(2):197-205.

Nanji AA, Zhao S, Lamb RG, Sadrzadeh SM, Dannenberg AJ, Waxman DJ. Changes in microsomal phospholipases and arachidonic acid in experimental alcoholic liver injury: relationship to cytochrome P-450 2E1 induction and conjugated diene formation. Alcohol Clin Exp Res. 1993 Jun;17(3):598-603.

Nanji AA, Zhao S, Lamb RG, Dannenberg AJ, Sadrzadeh SM, Waxman DJ. Changes in cytochromes P-450, 2E1, 2B1, and 4A, and phospholipases A and C in the intragastric feeding rat model for alcoholic liver disease: relationship to dietary fats and pathologic liver injury. Alcohol Clin Exp Res. 1994 Aug;18(4):902-8.

Fogt F, Nanji AA. Alterations in nuclear ploidy and cell phase distribution of rat liver cells in experimental alcoholic liver disease: relationship to antioxidant enzyme gene expression.Toxicol Appl Pharmacol. 1996 Jan;136(1):87-93.

Polavarapu R, Spitz DR, Sim JE, Follansbee MH, Oberley LW, Rahemtulla A, Nanji AA. Increased lipid peroxidation and impaired antioxidant enzyme function is associated with pathological liver injury in experimental alcoholic liver disease in rats fed diets high in corn oil and fish oil. Hepatology. 1998 May;27(5):1317-23.

Nanji AA, Fogt F, Griniuviene B. Alterations in glucose transporter proteins in alcoholic liver disease in the rat. Am J Pathol. 1995 Feb;146(2):329-34.

Li CJ, Nanji AA, Siakotos AN, Lin RC. Acetaldehyde-modified and 4-hydroxynonenal-modified proteins in the livers of rats with alcoholic liver disease. Hepatology. 1997 Sep;26(3):650-7.

Nanji AA, Dannenberg AJ, Jokelainen K, Bass NM. Alcoholic liver injury in the rat is associated with reduced expression of peroxisome proliferator-alpha (PPARalpha)-regulated genes and is ameliorated by PPARalpha activation. J Pharmacol Exp Ther. 2004 Jul;310(1):417-24.

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Wimberly AL, Forsyth CB, Khan MW, Pemberton A, Khazaie K, Keshavarzian A. Ethanol-induced mast cell-mediated inflammation leads to increased susceptibility of intestinal tumorigenesis in the APC Δ468 min mouse model of colon cancer. Alcohol Clin Exp Res. 2013 Jan;37 Suppl 1:E199-208.

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Morimoto M, Zern MA, Hagbjörk AL, Ingelman-Sundberg M, French SW. Fish oil, alcohol, and liver pathology: role of cytochrome P450 2E1. Proc Soc Exp Biol Med. 1994 Nov;207(2):197-205.

Tsukamoto H, Towner SJ, Ciofalo LM, French SW. Ethanol-induced fibrosis rats fed high fat diet. Hepatology 1986, 6:814-822

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Nanji AA, French SW. Dietary factors and alcoholic cirrhosis.Alcohol Clin Exp Res 1986, 10:271-273

Nanji AA, French SW. Relationship between pork consumption and cirrhosis. Lancet 1985, 1:681-683

Nanji AA, Mendenhall CL, French SW. Beef fat prevents alcoholic liver disease in the rat. Alcohol Clin Exp Res 1989, 13:15-19

Nanji AA, French SW. Dietary linoleic acid is required for development of experimental alcoholic liver disease. Life Sci 1989, 44:223-227

French SW: Nutrition in the pathogenesis of alcoholic liver disease. Alcohol Alcoholism 1993, 28:97-109 French SW. Miyamoto K, Tsukamoto H: Ethanol-induced fibrosis in the rat: role of amount of dietary fat. Alcohol Clin Exp Res 1986, 10:13S-19S

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有困惑?那就商量呗!

液体饲料的优点,你可以充分运用!

---------《》-------

marker可以任意缺乏或过载某营养素

marker可以任意添加药物或测试成分

marker可以精确定量饲料摄入量

marker可以任意定制饲料


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