动脉粥样硬化模型
豚鼠(荷兰猪)动脉粥样硬化模型饲料
和造模注意事项
Model Diet of Atherosclerosis in
Guinea-Pig
虽然有时把豚鼠归类于小型啮齿动物，但是，豚鼠的血脂代谢与大鼠、小鼠等差别较大，非常接近人类，用降血脂药物干预时，豚鼠的血脂也表现为类似人类的反应。因此，豚鼠被常用于动脉粥样硬化研究或者造模。
一、豚鼠动脉粥样硬化模型造模的特点
豚鼠用于动脉粥样硬化研究的优点：
(1)豚鼠对模型饲料中胆固醇敏感，能够发生动脉粥样硬化。
(2)豚鼠的血脂对模型饲料中脂肪类型或不同脂肪酸比例比较敏感，高脂高胆固醇模型饲料容易诱发动脉粥样硬化。
(3)豚鼠用高胆固醇模型饲料或者高脂高胆固醇模型饲料喂养几个月后能够到达动脉粥样硬化的斑块阶段（泡沫细胞，脂质沉积，水肿，等）。
(4)豚鼠与人类相似，需要依靠维生素Ｃ补充，有利于研究维生素C与动脉粥样硬化发生和发展的关系。
(5)豚鼠动脉粥样硬化模型适合降血脂和抗动脉粥样硬化药物活性的筛选和作用机制的研究。
豚鼠用于动脉粥样硬化研究的缺点：
动脉粥样硬化难以达到高级阶段。
二、豚鼠动脉粥样硬化造模的难点和动物要求
豚鼠对饲料中多种成分敏感甚至很敏感，无论是对照饲料还是模型饲料的配制，都是对饲料配比的考验。
豚鼠对饲料中成分的敏感性到达什么程度呢？饲料中蛋白质、碳水化合物、脂肪三者的含量，脂肪类型和不同脂肪酸的比例，维生素Ｃ的含量，等等，都决定着豚鼠血浆脂蛋白、甘油三酯和胆固醇浓度，因此：
(1)豚鼠的对照饲料必须科学配制。
(2)豚鼠动脉粥样硬化模型饲料必须严格控制和科学配比各项成分。
除了对照饲料和模型饲料，豚鼠本身的性别和年龄也决定动脉粥样硬化的发生和发展。一般来说，应当优先使用雄性、成年或接近成年的豚鼠。
三、豚鼠动脉粥样硬化模型饲料
豚鼠动脉粥样硬化模型饲料包括：高胆固醇模型饲料，高脂高胆固醇模型饲料。
1.豚鼠动脉粥样硬化高胆固醇模型饲料
豚鼠高胆固醇模型饲料中胆固醇含量必须达到一定高度，但是，过高可以导致某些器官（例如，肾脏）损伤。
用于豚鼠动脉粥样硬化模型的高胆固醇模型，包括两类：日粮型和纯化型。
日粮型的价格较低，但纯化型更适合豚鼠动脉粥样硬化模型。下表列出的是南通特洛菲饲料科技有限公司提供的豚鼠动脉粥样硬化高胆固醇模型饲料
豚鼠高胆固醇模型饲料
日粮型
以下模型饲料是在本公司豚鼠标准饲料(低脂)生产过程中添加胆固醇，其正常对照饲料应当选用本公司豚鼠标准饲料。
产品代码 模型饲料名称 说明
TP3G002：豚鼠高胆固醇模型饲料，0.2%胆固醇
TP3G003：豚鼠高胆固醇模型饲料，0.25%胆固醇
TP3G005：豚鼠高胆固醇模型饲料，0.33%胆固醇
TP3G008：豚鼠高胆固醇模型饲料，0.5%胆固醇
TP3G010：豚鼠高胆固醇模型饲料，1%胆固醇
如果以上模型饲料不能满足你的要求，请提出！
纯化型
以下模型饲料是纯化日粮，价格相对较高。
产品代码 模型饲料名称 说明
TP3G200：豚鼠高胆固醇模型饲料，0.25%胆固醇
TP3G204：豚鼠高胆固醇模型饲料，0.5%胆固醇
如果以上模型饲料不能满足你的要求，请提出！
2.豚鼠动脉粥样硬化高脂高胆固醇模型饲料
豚鼠对高脂饮食比较敏感，但是，应当注意，高脂高胆固醇模型饲料中脂肪类型和脂肪含量需要适度控制，因为在长期喂养过程中容易形成脂肪肝。下表列出的是南通特洛菲饲料科技有限公司提供的豚鼠动脉粥样硬化高脂高胆固醇模型饲料。
豚鼠高脂高胆固醇模型饲料
日粮型
以下模型饲料是在本公司豚鼠标准饲料(低脂)生产过程中添加脂肪和胆固醇，其正常对照饲料应当选用相应的低脂饲料。
产品代码 模型饲料名称 说明
TP3G300：豚鼠高脂高胆固醇模型饲料，10%脂肪，0.17%胆固醇
TP3G305：豚鼠高脂高胆固醇模型饲料，10%脂肪，0.35%胆固醇
TP3G310：豚鼠高脂高胆固醇模型饲料，15%脂肪，0.25%胆固醇
如果以上模型饲料不能满足你的要求，请提出！
纯化型
以下模型饲料是纯化日粮，价格相对较高。
产品代码 模型饲料名称 说明
TP3G400：豚鼠高脂高胆固醇模型饲料，35%(热量）脂肪，0.25%胆固醇
如果以上模型饲料不能满足你的要求，请提出！
对于上述描述中有不清楚之处或者其他注意事项，请与南通特洛菲饲料科技有限公司技术部联系。
References：
Alving, C. R. and G. M. Swartz, Jr. "Antibodies to Cholesterol, Cholesterol Conjugates and Liposomes: Implications for Atherosclerosis and Autoimmunity." Crit Rev Immunol 10, no. 5 (1991): 441-53.
Berger, F. M., J. F. Douglas, B. J. Ludwig and S. Margolin. "The Effect of N-Gamma-Phenylpropyl-N-Benzyloxy Acetamide (W-1372) on Experimental Atherosclerosis and Hypercholesteremia." J Pharmacol Exp Ther 170, no. 2 (1969): 371-81.
Bonner, M. J., B. F. Miller, H. V. Kothari and D. Kritchevsky. "Lysosomal Enzymes in Aortas of Species Susceptible and Resistant to Atherosclerosis." Proc Soc Exp Biol Med 139, no. 4 (1972): 1359-62.
Bozhko, GKh, L. S. Kostiukovs'ka, V. M. Kulabukhov and P. V. Voloshyn. "[Comparison of the Content of Cholesterol, Lipoproteins and Serum Proteins in Rats, Guinea Pigs and Rats for a Atherosclerosis Model]." Ukr Biokhim Zh 62, no. 5 (1990): 25-9.
Caravaca, J., E. G. Dimond, S. C. Sommers and R. Wenk. "Prevention of Induced Atherosclerosis by Peroxidase." Science 155, no. 3767 (1967): 1284-7.
Demireva, K. and I. Popdimitrov. "[Serum Lipids in Experimental Atherosclerosis in Guinea Pigs Treated with Protein Hydrolysate]." Eksp Med Morfol 16, no. 2 (1977): 64-7.
Dock, W. "Atherosclerosis. Why Do We Pretend the Pathogenesis Is Mysterious?" Circulation 50, no. 4 (1974): 647-9.
Ertepinar, H., B. Suzen, A. Ozoran, Y. Ozoran, S. Ceylan, G. Yeginoglu and G. Uremek. "Effects of Drag Reducing Polymer on Atherosclerosis." Biorheology 27, no. 5 (1990): 631-44.
Fernandez, M. L. and J. S. Volek. "Guinea Pigs: A Suitable Animal Model to Study Lipoprotein Metabolism, Atherosclerosis and Inflammation." Nutr Metab (Lond) 3, (2006): 17.
Fujinami, T., K. Okado, K. Senda, M. Sugimura and M. Kishikawa. "Experimental Atherosclerosis with Ascorbic Acid Deficiency." Jpn Circ J 35, no. 12 (1971): 1559-65.
Gaton, E. and M. Wolman. "Macrophage Activation in the Prevention or Regression of Atherosclerosis." Adv Exp Med Biol 168, (1984): 15-36.
Ginter, E. "Atherosclerosis and Vitamin C." Lancet 1, no. 7762 (1972): 1233-4.
Ginter, E., J. Babala, P. Bobek and Z. Dumbalova. "Model of Experimental Hypercholesterolaemia and Atherosclerosis in the Guinea-Pig." Cor Vasa 10, no. 2 (1968): 126-34.
Gryglewski, R. J., S. Chlopicki, J. Swies and P. Niezabitowski. "Prostacyclin, Nitric Oxide, and Atherosclerosis." Ann N Y Acad Sci 748, (1995): 194-206; discussion 206-7.
Hauss, W. H., U. Gerlach, G. Junge-Hulsing, H. Themann and W. Wirth. "Studies on the "Nonspecific Mesenchymal Reaction" and the "Transit Zone" in Myocardial Lesions and Atherosclerosis." Ann N Y Acad Sci 156, no. 1 (1969): 207-18.
Hogg, S. I. and A. Cryer. "Analysis and Lipoprotein Lipase Activation Capacity of Plasma Lipoproteins Isolated from Atherosclerosis-Susceptible White Carneau Pigeon and Atherosclerosis-Resistant Show Racer Pigeon." Comp Biochem Physiol B 79, no. 2 (1984): 251-4.
Ivanov, V. N. and L. P. Nikitina. "[Effect of Sea Buckthorn Oil on Certain Indices of Lipid Metabolism in Experimental Atherosclerosis]." Vopr Pitan, no. 6 (1973): 13-6.
Iwanaga, Y., A. Tanimura, H. Kitsukawa, J. Tanigawa and M. Aihara. "The Role of Endothelial Cells in the Pathogenesis of Atherosclerosis." Acta Pathol Jpn 19, no. 2 (1969): 161-78.
Kalachaveedu, M., S. Kuruvilla and K. Balakrishna. "Effect of Erythrina Variegata on Experimental Atherosclerosis in Guinea Pigs." J Pharmacol Pharmacother 2, no. 4 (2011): 285-7.
Klimov, A. N., T. A. Sinitsyna and V. A. Nagornev. "[Development of the Ideas of N. N. Anichkov at the Present Stage of Our Knowledge About Atherosclerosis]." Arkh Patol 37, no. 11 (1975): 3-16.
Kunitomo, M., K. Takaoka, J. Matsumoto, H. Iwai and Y. Bando. "[Experimental Induction of Atherosclerosis in Guinea Pigs Fed a Cholesterol and Vitamin D2-Rich Diet]." Nihon Yakurigaku Zasshi 81, no. 4 (1983): 275-83.
Lacko, A. G., H. L. Rutenberg and L. A. Soloff. "Serum Cholesterol Esterification in Species Resistant and Susceptible to Atherosclerosis." Atherosclerosis 19, no. 2 (1974): 297-305.
Lavrov, V. P. "[the Effect of Matsesta Hydrogen Sulfide Baths on the Status of the Myocardium and Coronary Arteries in Experimental Atherosclerosis]." Vopr Kurortol Fizioter Lech Fiz Kult 33, no. 4 (1968): 313-6.
Lecanu, L., Z. X. Yao, A. McCourty, K. Sidahmed el, M. E. Orellana, M. N. Burnier and V. Papadopoulos. "Control of Hypercholesterolemia and Atherosclerosis Using the Cholesterol Recognition/Interaction Amino Acid Sequence of the Translocator Protein Tspo." Steroids 78, no. 2 (2013): 137-46.
Leite, J. O., U. Vaishnav, M. Puglisi, H. Fraser, J. Trias and M. L. Fernandez. "A-002 (Varespladib), a Phospholipase A2 Inhibitor, Reduces Atherosclerosis in Guinea Pigs." BMC Cardiovasc Disord 9, (2009): 7.
Ma, J. Y., Y. K. Shi and D. Z. Fang. "[Antiatherogenic Effect of Huajiao Volatile Oil on Experimental Atherosclerosis in Guinea Pigs]." Sichuan Da Xue Xue Bao Yi Xue Ban 36, no. 5 (2005): 696-9.
Maksudov, B. S. "[Thyrotropic Function of the Pituitary Gland in Experimental Atherosclerosis in Rabbits]." Kardiologiia 4, (1964): 27-30.
Mancini, M., G. B. Rossi, P. Oriente and A. Cali. "Possible Relationship between Aortic Acid Mucopolysaccharides and Species-Susceptibility to Experimental Atherosclerosis." Nature 207, no. 5002 (1965): 1206.
Mangathayaru, K., S. Kuruvilla, K. Balakrishna and J. Venkhatesh. "Modulatory Effect of Inula Racemosa Hook. F. (Asteraceae) on Experimental Atherosclerosis in Guinea-Pigs." J Pharm Pharmacol 61, no. 8 (2009): 1111-8.
Miller, B. F., F. P. Keyes and P. W. Curreri. "Serum Beta-Glucuronidase Activity of Various Species: Possible Correlation with Susceptibility to Atherosclerosis." Proc Soc Exp Biol Med 119, no. 4 (1965): 1129-33.
Mironchik, V. V. "[Vitamins and Atherosclerosis]." Vopr Pitan, no. 5 (1983): 3-9.
Moghadasian, M. H. "Experimental Atherosclerosis: A Historical Overview." Life Sci 70, no. 8 (2002): 855-65.
Mosina, I. V. and V. E. Ryzhenkov. "[Effect of L-Dopa on the Development of Experimental Hyperlipidemia and Atherosclerosis]." Biull Eksp Biol Med 91, no. 3 (1981): 270-1.
Murase, T., M. Okubo and M. Hara. "Does Chylomicronemia Cause Atherosclerosis?" Tohoku J Exp Med 163, no. 2 (1991): 129-34.
Orimo, H. and Y. Ouchi. "The Role of Calcium and Magnesium in the Development of Atherosclerosis. Experimental and Clinical Evidence." Ann N Y Acad Sci 598, (1990): 444-57.
Pertsovskii, A. I. and Gorislavets, II. "[Peculiarities of the Course of Experimental Atherosclerosis in Guinea Pigs under the Effect of Several Additional Factors]." Arkh Patol 29, no. 4 (1967): 43-6.
Popdimitrov, I. and K. Demireva. "[Distribution of Methionine-75se in Animals with Experimental Atherosclerosis Treated with a Protein Hydrolysate]." Eksp Med Morfol 14, no. 3 (1975): 128-32.
Pretolani, E., A. Loreti and V. Foschi. "[Possibilities of the Overlapping of Fibrinolytic and Elastolytic Activity in Experimental Cholesterine Atherosclerosis]." Arzneimittelforschung 17, no. 8 (1967): 986-91.
Radomski, M. W. and E. Salas. "Nitric Oxide--Biological Mediator, Modulator and Factor of Injury: Its Role in the Pathogenesis of Atherosclerosis." Atherosclerosis 118 Suppl, (1995): S69-80.
Ray, T., P. C. Maity, S. Banerjee, S. Deb, A. K. Dasgupta, S. Sarkar and A. K. Sil. "Vitamin C Prevents Cigarette Smoke Induced Atherosclerosis in Guinea Pig Model." J Atheroscler Thromb 17, no. 8 (2010): 817-27.
Rosenson, R. S. and E. Hurt-Camejo. "Phospholipase A2 Enzymes and the Risk of Atherosclerosis." Eur Heart J 33, no. 23 (2012): 2899-909.
Rossi, G. B., M. Mancini, P. Oriente, A. Vecchione, R. Vecchione, R. Cerqua and M. Cuzzupoli. "Comparative Studies on Serum Lipids and Histochemical Features of the Arterial Wall in Animal Species Differently Susceptible to Experimental Atherosclerosis." J Atheroscler Res 5, no. 6 (1965): 569-79.
Rossi, G. B., P. Oriente, M. Cuzzupoli, A. Vecchione and M. Mancini. "Failure to Induce Atherosclerosis in 'Triton' Hyperlipaemic Guinea Pigs." Nature 203, (1964): 416.
Satinder, A. K. Sarkar, S. Majumdar and R. N. Chakravarti. "Effect of Ascorbic Acid Deficiency on the Development of Experimental Atherosclerosis." Indian J Med Res 86, (1987): 351-60.
Shaffer, C. F. "Ascorbic Acid and Atherosclerosis." Am J Clin Nutr 23, no. 1 (1970): 27-30.
Shtereva, M. and K. Demireva. "[Effect of a Protein Hydrolysate on Enzymatic Activity in the Aorta Wall in Experimental Atherosclerosis]." Eksp Med Morfol 19, no. 4 (1980): 216-20.
Sinzinger, H., P. Clopath, K. Silberbauer and M. Winter. "Is the Variation in the Susceptibility of Various Species to Atherosclerosis Due to Inborn Differences in Prostacyclin (Pgi2) Formation." Experientia 36, no. 3 (1980): 321-3.
Sotnikov Iu, I. "[Comparative Characteristics of the Pathological Anatomy of Spontaneous Atherosclerosis in Representatives of Various Classes of Animals]." Arkh Patol 29, no. 9 (1967): 61-7.
Tararak, E. M. "[Reverse Development of Experimental Aortic Atherosclerosis in Guinea Pigs]." Biull Eksp Biol Med 67, no. 2 (1969): 118-21.
Thomas, W. A. and D. N. Kim. "Biology of Disease. Atherosclerosis as a Hyperplastic and/or Neoplastic Process." Lab Invest 48, no. 3 (1983): 245-55.
Titov, V. N. "[Receptor Endocytosis of Polyenic Acids: Sensitivity and Resistance to Atherosclerosis (Review of the Literature)]." Klin Lab Diagn, no. 1 (1999): 3-8.
Torres-Gonzalez, M., J. S. Volek, J. O. Leite, H. Fraser and M. Luz Fernandez. "Carbohydrate Restriction Reduces Lipids and Inflammation and Prevents Atherosclerosis in Guinea Pigs." J Atheroscler Thromb 15, no. 5 (2008): 235-43.
Verbeuren, T. J. "Experimental Models of Thrombosis and Atherosclerosis." Therapie 61, no. 5 (2006): 379-87.
Vidaver, G. A., A. Ting and J. W. Lee. "Evidence That Lysolecithin Is an Important Causal Agent of Atherosclerosis." J Theor Biol 115, no. 1 (1985): 27-41.
Weber, G. and P. Tosi. "Observations with the Scanning Electron Microscope on the Development of Cholesterol Aortic Atherosclerosis in the Guinea-Pig." Virchows Arch A Pathol Pathol Anat 353, no. 4 (1971): 325-32.
West, K. L., T. L. Zern, D. N. Butteiger, B. T. Keller and M. L. Fernandez. "Sc-435, an Ileal Apical Sodium Co-Dependent Bile Acid Transporter (Asbt) Inhibitor Lowers Plasma Cholesterol and Reduces Atherosclerosis in Guinea Pigs." Atherosclerosis 171, no. 2 (2003): 201-10.
Wojcicki, J., L. Samochowiec, M. Jaworska and A. Hinek. "A Search for a Model of Experimental Atherosclerosis: Comparative Studies in Rabbits, Guinea Pigs and Rats." Pol J Pharmacol Pharm 37, no. 1 (1985): 11-21.
Xiangdong, L., L. Yuanwu, Z. Hua, R. Liming, L. Qiuyan and L. Ning. "Animal Models for the Atherosclerosis Research: A Review." Protein Cell 2, no. 3 (2011): 189-201.
Ye, P., I. K. Cheah and B. Halliwell. "High Fat Diets and Pathology in the Guinea Pig. Atherosclerosis or Liver Damage?" Biochim Biophys Acta 1832, no. 2 (2013): 355-64.
Ziemlanski, S., M. Wartanowicz, K. Potrzebnicka and B. Panczenko-Kresowska. "Ascorbic Acid and Tocopherol Levels in the Organs and Serum of Guinea Pigs with Experimentally Induced Atherosclerosis." Acta Physiol Pol 40, no. 5-6 (1989): 552-7.