小鼠肝脏干细胞(卵圆细胞)诱导法(CDE模型饲料)
Choline-Deficient Ethionine-Supplemented （CDE）Diet-Induced Oval Cells from mouse Liver

在大鼠使用CDE诱导肝脏干细胞（hepatic progenitor cells）的方法已经比较成熟和稳定之时，小鼠一直没有比较好的方法，因为按照大鼠的剂量转换成小鼠的剂量后死亡率很高，一般不超过2周几乎全军覆没。降低乙硫氨酸浓度虽然能减少死亡甚至不死亡，但诱导的肝脏干细胞非常少。而降低胆碱缺乏程度并且不改变乙硫氨酸浓度，则小鼠不仅状况良好而且在3周左右能够诱导出干细胞44%左右。

【饲料和方法】以下方法之一：

(1)采用含胆碱50%的胆碱缺乏模型饲料，饮水含乙硫氨酸0.15%

(2)购买无胆碱的胆碱缺乏模型饲料，饮水含乙硫氨酸0.15%

【乙硫氨酸】Sigma公司 No #E5139；Acros公司，等。

【实验动物】小鼠，年龄不限，但最常用成年或接近成年。

【喂养时间】3周或更长时间。

【注意事项】

参考文献

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