大鼠肝脏干细胞(卵圆细胞)诱导法(CDE模型饲料)
Choline-Deficient Ethionine-Supplemented （CDE）Diet-Induced Oval Cells from Rat Liver

大鼠是最先采用CDE模型饲料诱导肝脏干细胞( hepatic stem/progenitor cells)的动物。该法采用乙硫氨酸(DL-ethionine)损伤肝细胞，引起的损伤性反应中包括干细胞的反应性增加，从而通过分离、纯化、鉴定而获得肝脏的干细胞。

文献中一般采用以下3种方法之一：

(1)含0.1%乙硫氨酸的胆碱缺乏饲料（CDE模型饲料）

(2)胆碱缺乏饲料，饮水中加乙硫氨酸

乙硫氨酸一般用0.1%, 0.15%,。

(3)胆碱缺乏饲料，灌胃。

剂量是：按照动物每天饲料饲料量计算，相当于饲料量的0.05～0.1%，或者每天每kg体重50-70mg。(Kaplanski,等)

【乙硫氨酸】Sigma公司 No #E5139；Acros公司，等。

【实验动物】大鼠，年龄不限，但最常用成年或接近成年。

【喂养时间】6周左右。

【注意事项】

参考文献

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